Fig. 5.

DCs derived from different types of precursors were shown to exert opposite effects on effector cells in MLC after the challenge with anti-CD3/CD28 beads. Responders were effector cells rich in T-lymphocytes isolated either from peripheral blood or from cord blood. DCs before culture were stimulated for 24 h with either LPS (100 μg/ml) (❑) or IFN-γ (10 ng/ml) (❍). DCs were mixed with effectors in the following ratios – 1 : 0 and 0 : 1 (controls), 2 : 1, 1 : 1, 1 : 2, 1 : 4, and 1 : 8. The controls S and 2S contained effectors stimulated with either single or double doses of anti-CD3/CD28 beads. The results are presented as medians and minimum–maximum (* – statistical significance, ANOVA Kruskal-Wallis test, p < 0.05)