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. 2018 Dec 18;9:2896. doi: 10.3389/fimmu.2018.02896

Figure 3.

Figure 3

The presence of K14.E7xRbΔL/ΔL and K14.E7 DCs from grafted skin in the draining lymph node. Skin-derived DC in graft draining and control lymph node of CD45.1 recipients receiving K5mOVA, C57BL/6, K14.E7, K14.E7xRbΔL/ΔL, or RbΔL/ΔL (CD45.2) skin grafts. (A) experimental outline. Draining lymph nodes (axillary and iguanual) were harvested 11 days after grafting from either grafted side or non-grafted side of one graft recipient. (B) representative plots showing graft-derived (CD45.1–) DC under live CD45.1–gate. (C) quantitative result of migratory DC [vertical oblong gate in (B)] in grafted draining lymph node of recipients receiving indicated type of skin grafts. Plot showing the number of cells per 100,000 live cell analyzed. (D) number of cells per 100,000 live cells analyzed of graft-derived MigDC subtypes (CD103+, CD11b+ and LC) from lymph nodes of mice receiving indicated skin grafts. (E) MFI (top) and percentage (bottom) of CD80 and CD86 positive cells of various DC subtypes in grafted-drianing lymph nodes receiving either K14.E7 (closed circle) or K14.E7xRbΔL/ΔL (opened circle) skin graft. All statistics were done with one-way ANOVA with Bonferroni post-test. Plots show mean value with SEM. Result significance was shown, where *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.