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. 2018 Dec 18;9:2896. doi: 10.3389/fimmu.2018.02896

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Copyright © 2018 Kuo, Teoh, Tuong, Leggatt, Mattarollo and Frazer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Keratinocytes from K14.E7xRb ^ΔL/ΔL skin present endogenous antigen and are susceptible to CTL-mediated lysis. Keratinocytes isolated from the epidermis of C57BL/6, K14.E7, K14.E7xRb^ΔL/ΔL, and Rb^ΔL/ΔL mice were pulsed with (A) GF001 peptide (B) SIINFEKL peptide or (C) without peptide (-) and co-cultured with E7-specific effector T cells (E7TCR269) at the shown effector/target (E/T) ratios. Keratinocyte death was assessed as LDH release above background from no-peptide pulsed KC and effector T cell alone. Two independent experiments were done. Target cell maximum release and spontaneous release of LDH are plotted in Supplementary Figure 4. Data analyzed using two-way ANOVA and applied with Bonferroni post-test. Error bars showing mean ± SEM with 3 to 5 biological replicates in each experimental group. Result significance was shown, where ^****p < 0.0001.