Figure 13.

Fast electrophoresis of the amplification product of the PAX6 target fragments. B01 to B03 are target fragments of exon10 that DNA extracted from blood of proband's father (II2, Family 1); B04 to B06 are target fragments of exon10 that DNA extracted from semen of proband's father (II2, Family 1); B07 to B09 are target fragments of exon10 that DNA extracted from blood of proband (III5, Family 1). Their TMs were set as 56°C,58°C, and 61°C, respectively. B10 to B12 are target fragments of exon12 that DNA extracted from blood of proband's father (II2, Family 1), semen of proband's father (II2, Family 1), and blood of proband (III5, Family 1), respectively. The TM was set as 62°C