Table 6.
Pairwise comparisons of FANCD2 gene expression in two Fanconi anemia (FA) samples and two FANCD2 RV‐corrected FA samples
| Sample 1 | Sample 2 | Sample 1 FPKM | Sample 2 FPKM | log2 (Fold change) | FDR |
|---|---|---|---|---|---|
| FA1 | FA2 | 4.6 | 3.4 | −0.4 | 5.320 |
| FA1 | FA_RV1 | 4.6 | 108.6 | 4.5 | 0.000 |
| FA2 | FA_RV1 | 3.4 | 108.6 | 5.0 | 0.000 |
| FA1 | FA_RV2 | 4.6 | 124.2 | 4.7 | 0.000 |
| FA2 | FA_RV2 | 3.4 | 124.2 | 5.1 | 0.000 |
| FA_RV1 | FA_RV2 | 108.6 | 124.2 | 0.1 | 4.870 |
FPKM is the fragments per kilobase of exon per Million fragments mapped; FDR is false discovery rate.
Gene expression calculated by FPKM confirms that compared to FA samples, the expression of FANCD2 in the FA_RV samples is significantly increased. This verifies the retroviral correction of the FANCD2 gene in the FA_RV samples.