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. 2018 Nov 20;14:90–100. doi: 10.1016/j.omtn.2018.11.004

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© 2018 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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The Effect of JAZF1 Silencing on DNA Synthesis and Apoptosis of Human SSCs

(A–C) EDU incorporation assay illustrated the percentages of EDU-positive cells affected by control siRNA (A and C) and JAZF1-siRNA3 (B and C) in the human SSC line. Scale bars in (A) and (B), 10 μm. (D and E) Annexin V/PI staining and flow cytometry showed the percentages of early apoptosis (D and E, left) and late apoptosis (D and E, right) in human SSC line treated with JAZF1-siRNA3 and control siRNA. (C and E) The asterisk indicates statistically significant differences (p < 0.05) in human SSC line between JAZF1-siRNA3 and the control siRNA.