Figure 6.

Levitation of diamagnetic cells with negative magnetophoresis; patterning of pre-formed spheroids into the tissue strings and in situ 3D cellular assembly. (A) Serial coding of spatially controlled spheroids. Spheroids were formed separately and then inserted into the levitation device (spheroids; R, red; G, green; B, blue) Scale bars, 100 μm. Reprinted from Tocchio et al. (2017). Copyright (2017) John Wiley & Sons, Inc. (B) Cellular assembly of D1 ORL UVA^eGFP and MDA-MB-231^dsRed cells under microgravity. Confocal and conventional fluorescence microscopy (upper left) images showing self-assembled coculture clusters formed with magnetic levitation (100 mM Gd-BT-DO3A) and different cell loading strategies; L1: simultaneously loading of MDA-MB-231^dsRed and D1 ORL UVA^eGFP cells, L2: MDA-MB-231^dsRed cells onto D1 ORL UVA^eGFP clusters formed with magnetic levitation and L3: D1 ORL UVA^eGFP cells onto MDA-MB-231^dsRed clusters formed with magnetic levitation (total 50,000 cells/culture chamber). Scale bars: 200 μm. Reprinted from Anil-Inevi et al. (2018).