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. 2018 Dec 19;9:2980. doi: 10.3389/fimmu.2018.02980

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Copyright © 2018 Liao, Song, Gao, Mauer, Revzin and Malhi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Intrahepatic macrophages express the S1P₁ receptor. (A) CD45 expression and (B) F4/80 and S1P₁ receptor expression of CD45^hi intrahepatic macrophages was determined in normal mouse livers by FACS analysis. (C) S1P₁ receptor immunohistochemistry was performed in livers from mice fed a diet high in fat, fructose and cholesterol (FFC) or chow for 20 weeks. Scale bar = 50 μM, n = 4 for chow and n = 6 for FFC. (D) Quantification of immunohistochemistry for S1P₁ receptor in chow fed (n = 4) and FFC fed (n = 6) mice. (E) mRNA expression for S1P₁ receptor in chow fed (n = 5) and FFC fed (n = 5) mice. ^**p < 0.01.