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. 2018 Dec 19;9:3106. doi: 10.3389/fmicb.2018.03106

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Copyright © 2018 de Moraes, Barateiro, Sousa and Penha-Gonçalves.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Localization of B2R protein expression in placentas and cultured trophoblasts. (A) Immunohistochemistry of infected placentas showing B2R expression in mononuclear trophoblasts protruding to MBS (arrow; I and II) and in (B) Coan-Burton bridges (arrowhead; I and II); scale bar: 100 μM; (C) Immunofluorescence analysis of cultured trophoblasts from NI placentas; trophoblasts were stained with anti-KRT7-PE (green), anti-B2R-Cy5 (red), and DAPI (blue) and analyzed by fluorescence microscopy (Leica DMRA2); scale bar: 40 μM. The area depicted in the figure is magnified to show individual and merged staining; arrow shows B2R nuclear expression which was confirmed by (D) orthogonal views of stack imaging (acquired by Leica High Content Screening microscope) using ImageJ program; Cy, cytoplasm; N, nucleus.