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. 2018 Dec 19;9:2978. doi: 10.3389/fimmu.2018.02978

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Copyright © 2018 Landsberg, Megger, Hotter, Rückborn, Eilbrecht, Rashidi-Alavijeh, Howe, Heinrichs, Sauter, Sitek, Le-Trilling and Trilling.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Viral DDB1-interacting accessory proteins co-precipitate DDB1 with distinct efficiencies. Based on the MS data set (see Figure 1B), spectral indexes were calculated. (A) The abundance of DDB1 was calculated as fold over background based on the spectral indexes. (B) The co-precipitation of DDB1 was further analyzed for the herpesviral DDB1-binding proteins by IP and subsequent immunoblotting. The spectral indexes for Cul4B and Cul4A are depicted in (C,D), respectively. (E) The co-precipitation of DDB1 with the hepadnaviral DDB1-binding proteins was analyzed by IP and subsequent immunoblotting. (F) The DDB1 interaction was further analyzed by a reverse approach in which Flag-tagged DDB1 was expressed together with the viral accessory proteins. Lysates were generated, subjected to IP using an α-Flag antibody, and analyzed by immunoblotting.