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. 2018 Dec 3;202(1):183–193. doi: 10.4049/jimmunol.1800636

FIGURE 1.

FIGURE 1.

LACC1 is expressed in myeloid cells and is LPS inducible. (A) LACC1 protein levels in primary mouse macrophages (MΦ), dendritic cells (DC), or neutrophils (Neu) isolated from LACC1 KO or WT mice as assessed by Western blotting of total cell lysates (10 μg) with polyclonal Abs to LACC1 or mAbs to GAPDH. (B) LACC1 mRNA expression relative to HPRT levels in BM-DCs in WT or LACC1 KO cells untreated or stimulated with 100 ng/ml LPS, 5 μM CPG (ODN1826), or S. typhimurium flagellin (Fla) for 24 h and a time course for BM-mac. Data from two independent experiments (n = from 6 to 9 mice) or one experiment [BM-DC with CPG/FLA (n = 3) and WT BM-mac with LPS for 2 h/24 h (n = 4)]. Error bars show SD. (C) Protein levels of LACC1 assessed by Western blotting 24 h after LPS or mock treatment, quantification on the right. Positive control, HEK cells transfected with a LACC1 expression construct (Myc-DDK–tagged); negative control, mock-transfected HEK cells. *p < 0.05, **p < 0.01, ***p < 0.00.