FIGURE 3.

Enhanced CIA in LACC1 KO mice. LACC1 KO (red) and age-matched WT control (black) mice were immunized with 200 μg of chicken type II collagen in CFA intradermally on day 0 and day 21. (A) Paw arthritis scores were monitored over time. Data are presented as mean ± SEM from two independent experiments (n = 29–39 mice per timepoint). (B) At day 40, all paws (left) or arthritic paws (right) were histologically scored for lesion severity from all the mice in one of the experiments (n = 9 WT and 13 LACC1 KO mice). Data are presented as mean ± SD. (C) Number of arthritic animals and paws at day 20 (top two rows) and day 48 (bottom two rows) after initial collagen immunization. (D) Arthritis score from arthritic mice at day 20 CIA, data combined from three independent experiments (n = 19 WT and 25 KO). Data presented as mean ± SD. (E and F) Arthritis scores and numbers of affected paws normalized to arthritis onset. Data represent 18–30 mice in each group per timepoint and are presented as mean ± SEM. (G) The individual arthritis score for each paw normalized to the individual paw arthritis onset over time. Data combined from two independent experiments and presented as mean ± SEM. (n = 55–95 paws per timepoint). *p < 0.05, **p < 0.01.