FIGURE 5.

Dysregulation of TNF and IL-17 in LACC1 KO mice. (A) TNF mRNA expression relative to housekeeping genes in individual WT (black) or LACC1 KO (red) mice from multiple in vivo models. RNA was isolated from day 28 CIA paws with an arthritis score of 0 (left) or 1 (right), MIA paws at day 10, C. rodentium colons at day 13, and DSS colons at day 8. Data represent at least two independent experiments. (B) Serum TNF protein levels measured by Luminex at day 20, 28, or 48 CIA. (C) Day 20 CIA splenocytes (top) or cells from the draining inguinal lymph node (bottom) received no stimulation (left) or were stimulated with anti-CD3 and anti-CD28 Abs for 6 h (right) followed by intracellular staining for IL-17. Data are from one experiment harvested on day 20 or 22 (n = 12–18 mice per condition). (D) Splenocytes (top) and lymphocytes (bottom) from 5-mo-old mice were stimulated with 50 ng/ml PMA and 500 ng/ml ionomycin for 6 h followed by intracellular staining for IL-17 (left) or IFN-γ (right). Data are combined from three independent experiments (n = 10 per group). IL-17 data are shown as fold change relative to WT for each experiment due to experiment-to-experiment variability. Data in all panels in this paper are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.