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. 2018 Dec 21;12(3):462–474. doi: 10.1016/j.tranon.2018.11.014

Figure 5.

Figure 5

Generation of L-plastin and PRDX4 targeting shRNA clones. (A-D) MDA-MB-231 cells (231) were transfected with shRNA targeting L-plastin (shL), peroxiredoxin-4 (shP), a combination of both L-plastin and PRDX4 (shLP), or an empty vector (Luc), and stable clones were generated. Expression of L-plastin and PRDX4 in cell lysates (CL, A, B) and conditioned medium (CM, C, D) was assessed by immunoblotting. Calnexin and Ponceau stains were used as loading controls for CL and CM, respectively. Shown are representative immunoblots (A, C) and their quantification relative to loading control (B, D). Data are means ± SEM, N = 3 independent experiments for L-plastin and N = 1 for PRDX4.