Fig. 2.

The result of amplification of coding sequences and splicing by overlap extension-PCR reaction analyzed by gel electrophoresis. (A(PCR products of the Core and SP-Core coding sequences, (B) PCR products related to the step-by-step addition of the SP to the Core-coding sequence using SOE-PCR. F1-F4 indicate the PCR result of using primers F1-F4 (Table 2) to extend core sequence. PCR result of cloning confirmation of (C) GFP, (D) Core, and (E) SP-Core in pGE-30446-HCE using the forward primer of the M13 and the reverse primer of the core gene. M, marker