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Predicted probability of nucleotides being paired or single stranded using RNA structure program. Predicted probability of nucleotides of (A) synthesized backbone sequence RNA, (B) Core protein RNA, and (C) SP-Core RNA. Probability lower than 50% is not colored. The calculated minimum free energies for secondary structure of synthesized sequence alone and containing Core and SP-Core coding sequences were -307.3, -611.3, -647.2 kcal/mol, respectively.
