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. 2018 Sep 18;7(1):251–253.e1. doi: 10.1016/j.jcmgh.2018.09.007

Figure 2.

Figure 2

Immunofluorescence of foveolar cell or SPEM markers in Lrig1-Kras or Mist1-Kras mouse stomachs. (A) Sections of the stomach corpus from Lrig1-Kras or Mist1-Kras mice were co-stained for UEAI, GSII, and GIF. UEAI+ cells indicate foveolar cells, GSII+ cells are mucous neck cells, and GIF+ cells are mature chief cells. Dual GIF+/GSII+ cells indicate SPEM cells (white arrows). Dotted boxes indicate enlarged regions. (B) Sections of the corpus from Lrig1-Kras or Mist1-Kras mouse stomachs were immunostained with antibodies against Ki67, a proliferating cell marker, with UEAI and GIF. White arrows indicate proliferating SPEM cells at the base of metaplastic glands and dotted boxes indicate enlarged regions. Quantitation of GIF+/Ki67+ cells containing glands per 20× field in (C) Lrig1-Kras or (D) Mist1-Kras mouse stomach corpus. A total of 1.55% of glands contained GIF+/Ki67+ cells in Lrig1-Kras mouse corpus and 49.92% of glands contained GIF+/Ki67+ cells in Mist1-Kras mouse corpus. Error bars indicate SD (N = 3). (E) Sections of the stomach corpus from Lrig1-Kras or Mist1-Kras mice were co-stained for UEAI, CD44v9, and Clu. Cells with only UEAI staining indicate foveolar cells and dual CD44v9+/Clu+ cells indicate SPEM cells (white arrows). Dotted boxes indicate enlarged regions.