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. 2018 Aug 23;64(6):503–510. doi: 10.1262/jrd.2018-080

Fig. 1.

Fig. 1.

Oocyte-cumulus-granulosa complexes (OCGCs) before and after day 12 of the growth culture. A: An OCGC before the growth culture. The OCGC has an oocyte surrounded by a cumulus investment and attached mural granulosa-cell layer. The white arrowhead indicates the cumulus investment. The black arrowhead indicates the mural granulosa-cell layer. B: A surviving OCGC without antrum formation in the granulosa cell layer after a 12-day growth culture. OCGCs having oocytes with an evenly granulated ooplasm and enclosed by several layers of healthy granulosa cells were defined as surviving. C: A dead OCGC having a degenerated oocyte. D: A surviving OCGC with the formation of antra (white arrowheads) in the granulosa cell layer. The white arrow indicates an oocyte. Scale bars indicate 100 µm.