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. 2018 Sep 1;64(6):511–522. doi: 10.1262/jrd.2018-093

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©2018 Society for Reproduction and Development

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)

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Fig. 5. — Fertility of recipient mice after acyline treatment. (A) Offspring sired by acyline-treated recipient mice, showing fluorescence under UV light. (B) Macroscopic appearance of a fertile recipient testis transplanted with MHY-GS cells, showing extensive colonization. (C) Testis weight of acyline- and leuprolide-treated testes (n = 4). (D) Immunostaining of recipient testis that underwent acyline or leuprolide treatment. (E) Quantification of tubules with spermatogenesis. At least 271 tubules in four testes were counted. Bar = 1 mm (B), 50 μm (D). Asterisk indicates statistical significance (P < 0.05).