Figure 4.

Mutations in LUC7 Result in Misexpression of Protein-Coding and Noncoding Genes, but Not in MIRNA Genes.

(A) Differentially expressed genes from RNA-seq experiments with the wild type and luc7a luc7b luc7rl mutants were classified in protein coding genes, ncRNAs, miRNAs, and transposons (Supplemental File 1).

(B) RT-qPCR analysis of selected ncRNA in the wild type and luc7a luc7b luc7rl. Differentially expressed ncRNAs identified by RNA-seq were analyzed by RT-qPCR. Total RNAs were isolated from 7-d-old seedlings. Error bars denote se (n = 3).

(C) RT-qPCR analysis of four selected miRNAs in the wild type and luc7a luc7b luc7rl. Total RNAs were isolated from 7-d-old seedlings. Error bars denote se (n = 3).