Figure 5.

Global Analysis of Splicing Defects in luc7 Triple Mutant.

(A) Classification of splicing events changes in luc7 triple mutant compared with the wild type.

(B) to (F) Coverage plots and RT-PCR validation experiments for selected splicing events in the wild type and luc7 triple mutant. Genomic DNA (gDNA) or water (−) served as a control. Primer positions are indicated with gray arrows. IR, intron retention; ES, exon skipping; Alt.3ʹSS, alternative 3ʹ splicing site; Alt.5ʹSS, alternative 5ʹ splicing site.