Figure 7.

mRNAs Containing Retained LUC7-Dependent Introns Are NMD-Insensitive and Remain Nuclear.

(A) RT-PCR analysis of LUC7-dependent introns in the wild type and NMD mutants (lba1 and upf3-1).

(B) Splicing patterns of mRNAs isolated from total (T), cytosolic (C), and nuclear (N) fractions. RT-PCR analyses were performed using oligonucleotides flanking the LUC7-dependent splicing event.

(C) Immunoblot analysis of proteins isolated from total, cytosolic, and nuclear fractions. Blots were probed with antibodies against histone H3 and a ribosomal protein, L13-1.