Table 1. Selected Potential LUC7A Interacting Proteins Identified in Immunoprecipitation Experiments Followed by MS Analysis.
Identified Protein | Aver. No. of Peptides IP LUC7 | Aver. No. of Peptides IP Wild Type | Seq. Coverage (%) IP LUC7 | Seq. Coverage (%) IP Wild Type | MW (kD) |
---|---|---|---|---|---|
U1 snRNP components | |||||
LUC7A | 45.8 | 0 | 71.9 | 0 | 47.4 |
U1-70K | 5.0 | 0 | 13.3 | 0 | 50.4 |
SmB-a; SmB-b | 2.5 | 0 | 9.8 | 0 | 27.0 |
U1A | 2.0 | 0 | 7.7 | 0 | 28.1 |
Splicing-related | |||||
U2AF35A; U2AF35B | 3.5 | 1.0 | 11.9 | 4.3 | 34.6 |
SCL30A; SCL33 | 2.8 | 0 | 12.3 | 0 | 20.2 |
SR45 | 1.8 | 0 | 5.2 | 0 | 44.6 |
RSZ21; RSZ22; RSZ22A | 1.8 | 0 | 8.0 | 0 | 22.5 |
SR30 | 1.0 | 0 | 3.9 | 0 | 29.1 |
RS2Z33 | 1.0 | 0 | 2.9 | 0 | 32.9 |
SCL30 | 0.8 | 0 | 2.8 | 0 | 29.6 |
U2AF65A | 0.8 | 0 | 1.7 | 0 | 60.7 |
Kinases | |||||
SRPK4 | 8.5 | 0 | 16.2 | 0 | 59.4 |
SRPK3 | 4.3 | 0 | 8.9 | 0 | 61.2 |
Proteins linked to 3′ end processing/poly(A) binding | |||||
SPT6L (AT1g63210, AT1g65440) | 8.5 | 1.0 | 4.8 | 0.9 | 185.0 |
Poly(A)-binding protein RBP47C | 1.5 | 0.3 | 4.0 | 0.7 | 48.6 |
La-related protein 6B (LARP6) | 2.3 | 0.7 | 5.5 | 1.9 | 60.6 |