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. 2018 Oct 18;30(11):2779–2794. doi: 10.1105/tpc.18.00297

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Figure 4. — TOE1/2 Are Associated with Region F of the FLS2 Promoter.

(A) The partial structure of the FLS2 gene and regions amplified by ChIP-PCR.

(B) Enrichment of the indicated TOE1-associated DNA regions in protoplasts expressing TOE1-HA as determined by ChIP-PCR. TOE1-HA was transfected in protoplasts. Chromatin from protoplasts expressing TOE1-HA was immunoprecipitated with anti-HA antibodies, enrichment levels were normalized to those of protoplasts transfected with empty vectors, and no-antibody (NA) immunoprecipitates served as a control. Values are means ± sd of three biological replicates. Statistical significance compared with the NA control was determined by Student’s t tests: ***P < 0.001.

(C) Enrichment of the F region DNA of the FLS2 promoter in ProTOE1:TOE1-HA and ProTOE2:TOE2-HA transgenic plants. Chromatin from 2-d-old ProTOE1/2:TOE1/2-HA transgenic seedlings was immunoprecipitated with anti-HA antibodies, enrichment levels were normalized to those of Col-0 seedlings, and NA immunoprecipitates served as a control. Values are means ± sd (n = 3). Statistical significance compared with the NA control was determined by Student’s t tests: ***P < 0.001. Three independent replicates were performed, and similar results were obtained.