Figure 1.
Phenotypes of the rcd7 Mutant and Candidate Insertion Mutants and Allelism Tests.
(A) Representative photographs of 3-week-old O3-treated (350 ppb) and clean air (CA) control (<20 ppb) Col-0 gl1 and rcd7 plants taken 18 h after the end of a 6 h exposure to O3.
(B) Trypan blue staining for dead and dying cells performed 18 h after the end of O3 exposure.
(C) to (E) Electrolyte leakage measured 4, 18, and 26 h after the end of O3 exposure. Values are plotted as % of total ion content. At least three independent experiments consisting of four plants per line for each treatment were performed with similar results. Data from a representative experiment is shown. Data are presented as mean ± sd (n = 4 plants). Asterisks indicate statistically significant differences to O3-treated Col-0 gl1 (C and E) or Col-0 (D) (ANOVA with Tukey’s honestly significant difference [HSD] test, P < 0.05).