Figure 10.

In Vitro Phosphorylation Sites of GST-GHR1 by 6xHis-HT1 Identified by Mass Spectrometry

Purified recombinant GST-GHR1 was phosphorylated by 6xHis-HT1 in vitro and the phosphorylation sites were subsequently identified by mass spectrometry. The positions of all identified phosphorylation sites are depicted on the GHR1 protein structure (upper panel). Phosphorylation sites located on the intracellular juxtamembrane domain and pseudokinase domain (gray highlighting) are additionally marked (bold letters) on the GHR1 protein sequence (lower panel). SP, signal peptide; LRR, leucine-rich repeat; TM, transmembrane domain; KD, kinase domain. See Supplemental Data Set 2 for MaxQuant Phospho(STY)Sites table.