Figure 7.

Functional Domains of GHR1.

(A) to (D) Characterization of stomatal phenotypes of ghr1-17 and the F₁ progeny from its cross to ghr1-3 (GK_760C07) and Col-0 with GC1:YC3.6. (A) Stomatal conductance of intact plants. Data are presented as mean ± sem (n = 5–7 plants) and derive from two independent batches of plants. Significant differences (ANOVA with Tukey’s HSD test, P < 0.05) between lines are denoted with different letters. (B) to (D) Stomatal response of intact plants to (B) O₃ pulse, (C) elevated CO₂, (D) darkness. Stomatal conductance (expressed in relative units) of 3- to 4-week-old plants was recorded; at time point zero, the indicated treatments were applied. Data points represent means ± sem (n = 5–7 plants).

(E) Subcellular localization of 35S:GHR1:YFP, 35S:GHR1^W799*:YFP and 35S:YFP fusion proteins in Nicotiana benthamiana epidermal cells. Representative images for each construct taken with identical confocal microscopy acquisition settings are shown.

(F) Leaf fresh weight loss in 2 h and (G) stomatal conductance of intact plants in independent transgenic lines expressing 35S:GHR1:GFP or 35S:GHR1^KD:GFP in ghr1-3. Data are pooled from two independent experiments and are presented as mean ± sem (n = 6–10 plants in [F], n = 6-8 plants in [G]). Significant differences (ANOVA with Tukey’s HSD test, P < 0.05) between lines are denoted with different letters.