Skip to main content
. 2018 Dec 19;9:1823. doi: 10.3389/fphys.2018.01823

FIGURE 5.

FIGURE 5

NRF1 overexpression inhibits chicken preadipocyte differentiation. (A) Western blot analysis of NRF1 overexpression during chicken preadipocyte differentiation. ICP1 cells were transfected with either pCMV-HA-NRF1 or empty pCMV-HA vector as a control. At 24 h after transfection, the cells were induced to differentiate by adding 160 μM sodium oleate for 24, 48, and 72 h, and the cell lysates were harvested and immunoblotted with an HA-specific antibody. (B) The effects of NRF1 overexpression on lipid droplet accumulation during ICP1 cell differentiation. Oil Red O staining of the ICP1 cells transfected with pCMV-HA or pCMV-HA-NRF1 was performed at 72 h of differentiation (left two panels) and its quantification at 24, 48, and 72 h (right panel). Statistical significance was determined by Student’s t-test comparing empty pCMV-HA vector versus pCMV-HA-NRF1 transfection. ∗p < 0.05, ∗∗p < 0.01. (C) Real-time RT-PCR analysis of expression levels of PPARγ1 and adipogenic differentiation markers (FABP4, G0S2, GPDH, and AdipoQ) during the differentiation of ICP1 cells transfected with either empty pCMV-HA vector or pCMV-HA-NRF1 at the indicated times points. All data represent the mean ± SE. Statistical significance was determined by Student’s t-test comparing empty pCMV-HA vector versus pCMV-HA-NRF1 transfection. ∗p < 0.05, ∗∗p < 0.01.