Fig. 7.

S-mycothiolated OhsR bound to the ohr promoter DNA. a–c Binding of reduced OhsR, OhsR-SOH, and OhsR-SSM to the ohr promoter examined by EMSA. Interaction of the increasing amounts of OhsR with the ohr promoter was detected using gel shift experiments. BSA instead of OhsR (Last lane) in the binding assays was used as negative controls to determine the binding specificity of OhsR. d EMSA experiment of interactions between DTT-treated OhsR:C125S or CHP- and MSH-treated OhsR:C125S and the ohr promoter was determined as described above. Similar results were obtained in three independent experiments, and data shown are from one representative experiment done in triplicate