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. 2018 Oct 4;219(2):323–334. doi: 10.1093/infdis/jiy506

Figure 1.

Figure 1.

Monophosphoryl lipid A (MPL) + trehalose-6,6’-dicorynomycolate (TDCM) + squalene oil (MTS) increases primary and secondary pneumococcal polysaccharide (PPS)–specific immunoglobulin M (IgM) and immunoglobulin G (IgG) responses to Pneumovax. A–D, C57BL/6 wild-type mice were immunized with Pneumovax (~1 μg each PPS) alone or mixed with Sigma adjuvant system containing 20 μg MPL + 20 μg synthetic TDCM in 1% squalene oil (MTS) intraperitoneally (n = 4–5 mice/group) on days 0 and 30. Enzyme-linked immunosorbent assays to detect mean PPS3-specific (A and C) and Pneumovax (Px)–specific (B) serum IgM and IgG were performed. In C, PPS3-specific IgG levels were measured at 6 months postimmunization (n = 4–5 mice/group). D, Mean PPS3-specific IgM and IgG antibody-secreting cell frequencies in spleen and bone marrow (BM) 6 months postimmunization (n = 4–5 mice/group). *Significant differences between groups (P < .05). Results in A and B are representative of 3 independent experiments.