Figure 3.
Monophosphoryl lipid A (MPL) + trehalose-6,6’-dicorynomycolate (TDCM) + squalene oil (MTS) significantly increases immunoglobulin M (IgM) and immunoglobulin G (IgG) responses to haptenated-Ficoll. A and B, Mice were immunized with 1 μg trinitrophenyl (TNP)65-Ficoll (A) or nitrophenyl (NP)40-Ficoll (B) either alone or mixed with MTS intramuscularly and boosted on day 30. C, Mice were immunized intraperitoneally with 1 μg TNP-Ficoll alone or mixed with MTS. Thirty days later, mice were immunized with 25 μg TNP-Ficoll ± MTS and TNP-specific IgM and IgG levels were measured by enzyme-linked immunosorbent assay. *Significant differences between groups (n = 5–6 mice/group, P < .05).