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. 2018 Oct 4;219(2):323–334. doi: 10.1093/infdis/jiy506

Figure 4.

Figure 4.

Monophosphoryl lipid A (MPL) + trehalose-6,6’-dicorynomycolate (TDCM) + squalene oil (MTS) increases nitrophenyl (NP)–specific immunoglobulin M (IgM) and immunoglobulin G (IgG) antibody-secreting cell (ASC) frequencies in spleen and bone marrow (BM) and IgG+ memory cells. A, MTS increases long-lived NP-specific IgM and IgG ASCs. Mice were immunized with NP-Ficoll (1 μg) either alone or mixed MTS (n = 3 mice/group) and boosted on day 30. An enzyme-linked immunospot assay was performed to detect NP-specific IgM and IgG ASC frequencies in spleen and BM 3 months postimmunization. B, MTS significantly increases IgG switched memory cells following NP-Ficoll immunization. CD45.2+ wild-type mice received 1 × 107 VHB1-8 CD45.1+ transgenic CD43– spleen B cells intravenously. One day later, recipient mice were immunized with NP-Ficoll (1 μg) either alone or mixed with MTS, intramuscular (n = 4 mice/group). NP-specific CD45.1+ B cells were analyzed by flow cytometry 3 weeks postimmunization. *Significant differences between NP-Ficoll and NP-Ficoll + MTS (P < .05).