Table 2.

Synoptic report that at glance supply evidences regarding the microbial physiology of SeITE02 and MPV1 strains, as well as physical–chemical and biological features of selenium nanomaterials.

Stenotrophomonas maltophilia SeITE02		Ochrobactrum sp. MPV1
Growth of the environmental isolates under metabolically controlled conditions
• Glucose and pyruvate are the sources of carbon and energy exploited by bacterial cells to grow either in the presence or absence of as selenium precursor;
• Both strains can biotically convert synthesizing selenium nanomaterials
Occurrence of selenium nanostructures
• Intracellular		• Extracellular and on the outer cellular surface
Physiology features and morphology of the biogenic nanomaterial as function of the carbon source
Glucose	Pyruvate	Glucose	Pyruvate
• Microbial vesicles (MVs);		• SeNPs;	• Intracellular inclusions;
		• SeNRs	• SeNPs
• Extracellular Polymeric Substance (EPS);
• SeNPs
Elemental composition of the organic coatingof selenium nanostructures
• Carbon;		• Carbon;
• Oxygen;		• Oxygen
• Sulfur;
• Phosphorous
Macromolecules detected by fluorescence spectroscopy
• Proteins (λem = 325 nm);
• Amphiphilic molecules upon labeling with DiOC18(3) (λem = 507 nm)
Photoluminescence properties of selenium nanostructures
• Photoluminescence emission maxima (λem) at 416 nm (λexc = 380 nm);
• Red-shift of photoluminescence emission centered at 530 or 640 nm upon excitation of the biogenic nanomaterial at 485 or 532 nm, respectively;
• Linear dependency of the photoluminescence emission on the excitation wavelength