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. 2018 Dec 21;12:135–146. doi: 10.2147/OTT.S186607

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© 2019 Guo et al. This work is published and licensed by Dove Medical Press Limited

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DANCR knockdown inhibits the proliferation of NSCLC cells.

Notes: (A) qRT-PCT analyses of DANCR expression in Ctrl and DANCR shRNA-transfected A549 cells. (B) Cell counting assay for the growth of A549 cells in Ctrl and DANCR knockdown groups. (C) Colony formation assays for A549 cells in Ctrl and DANCR knockdown groups. (D) Flow cytometric analyses of cell apoptosis in Ctrl and DANCR shRNA-transfected A549 cells. (E) Cell cycle distribution of Ctrl and DANCR shRNA-transfected A549 cells was determined by flow cytometry. (F) qRT-PCR of cell cycle- and apoptosis-related genes in Ctrl and DANCR shRNA-transfected A549 cells. (G) Western blot assays for the expression of cell cycle- and apoptosis-related proteins in Ctrl and DANCR shRNA-transfected A549 cells. ^*P<0.05, ^**P<0.01, ^***P<0.001.

Abbreviations: Ctrl, control; NSCLC, non-small-cell lung cancer; PI, propidium iodide; qRT-PCR, quantitative RT-PCR.