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. 2018 Dec 21;12:135–146. doi: 10.2147/OTT.S186607

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© 2019 Guo et al. This work is published and licensed by Dove Medical Press Limited

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DANCR overexpression promotes the proliferation of NSCLC cells.

Notes: (A) qRT-PCT analyses of DANCR expression in Ctrl and DANCR-overexpressing plasmid-transfected H1299 cells. (B) Cell counting assay for the growth of H1299 cells in Ctrl and DANCR overexpression groups. (C) Colony formation assays for H1299 cells in Ctrl and DANCR overexpression groups. (D) The cell cycles of H1299 cells with DANCR overexpression were analyzed by flow cytometry. (E) Cell apoptosis in H1299 cells transfected with DANCR was determined by flow cytometry. (F) qRT-PCR analyses of Bcl-2, Bax, Cyclin D1, and p21 gene expression in DANCR-overexpressing H1299 cells. (G) Western blot analyses for Bcl-2, Bax, Cyclin D1, and p21 proteins in DANCR-overexpressing H1299 cells. ^*P<0.05, ^**P<0.01, ^***P<0.001.

Abbreviations: NSCLC, non-small-cell lung cancer; qRT-PCR, quantitative RT-PCR.