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. 2018 Dec 21;12:135–146. doi: 10.2147/OTT.S186607

Figure 6.

Figure 6

DANCR epigenetically silences p21 expression to promote NSCLC cell proliferation, migration, and invasion.

Notes: (A) RIP assays for the binding of DANCR and EZH2. (B) ChIP assays for the binding of EZH2 to p21 gene and the levels of H3K27me3 in p21 gene in Ctrl and DANCR knockdown groups. (C) qRT-PCR analyses of DANCR and p21 expression in A549 cells from Ctrl, DANCR knockdown alone, and p21 siRNA co-transfection groups. (D) Cell counting and (E) colony formation assays for A549 cells in Ctrl, DANCR knockdown alone, and p21 siRNA co-transfection groups. (F) Transwell migration assay and Matrigel invasion assay for A549 cells in Ctrl, DANCR knockdown alone, and p21 siRNA co-transfection groups. **P<0.01, ***P<0.001.

Abbreviations: ChIP, chromatin immunoprecipitation; Ctrl, control; NSCLC, non-small-cell lung cancer; RIP, RNA immunoprecipitation; qRT-PCR, quantitative RT-PCR.