Table S1.
Physicochemical properties of SPIONLA-HSA and SPIONLA-HSA-Ptx particles
| Parameter | SPIONLA-HSA | SPIONLA-HSA-Ptx |
|---|---|---|
|
| ||
| Hydrodynamic size in H2O (day 1/day 8) (nm) | 73.6±1.9/70.4±2.9 | 72.8±2.0/71.4±0.8 |
| Hydrodynamic size in RPMI (0% FBS) (day 1/day 8) (nm) | 58.5±1.1/55.5±0.3 | 58.3±1.6/55.6±0.3 |
| Hydrodynamic size in RPMI (10% FBS) (day 1/day 8) (nm) | 58.8±0.6/57.8±0.7 | 59.4±0.4/58.8±2.2 |
| Hydrodynamic size in DMEM (0% FBS) (day 1/day 8) (nm) | 57.8±0.9/58.7±0.9 | 57.7±1.3/58.6±0.6 |
| Hydrodynamic size in DMEM (10% FBS) (day 1/day 8) (nm) | 57.2±1.0/57.5±1.1 | 57.7±1.6/57.8±0.4 |
| PDI in H2O (day 1/day 8) | 0.180±0.009/0.194±0.002 | 0.185±0.011/0.176±0.013 |
| PDI in RPMI (0% FBS) (day 1/day 8) | 0.166±0.005/0.159±0.003 | 0.158±0.017/0.156±0.006 |
| PDI in RPMI (10% FBS) (day 1/day 8) | 0.267±0.002/0.268±0.005 | 0.267±0.005/0.272±0.008 |
| PDI in DMEM (0% FBS) (day 1/day 8) | 0.151±0.011/0.160±0.014 | 0.15±0.013/0.162±0.009 |
| PDI in DMEM (10% FBS) (day 1/day 8) | 0.260±0.002/0.263±0.007 | 0.263±0.001/0.278±0.005 |
| ζ Potential at pH 6.28*/6.65** (mV) | −11.2±0.9 | −13.2±2.9 |
| ζ Potential at pH ~4.0 (mV) | 21.7±1.9 | 23.0±0.6 |
| Isoelectric point (ζ=0 forward reaction) (pH) | 6.07±0.17 | 6.37±0.18 |
| Isoelectric point (ζ=0 backward reaction) (pH) | 4.95±0.10 | 4.97±0.05 |
| Magnetization at 5 T (kA/m) | 467±15 | 465±15 |
Notes: Summary of the main physicochemical properties of SPIONLA-HSA and SPIONLA-HSA-Ptx particles. The hydrodynamic diameter and PDI of newly prepared particles were measured at day 1 and additionally after 7 days of storage at 4°C.
Zeta potential measurement of SPIONLA-HSA at the pH value of freshly prepared particles.
Zeta potential measurement of SPIONLA-HSA-Ptx at the pH value of freshly prepared particles.
Abbreviations: DMEM, Dulbecco’s Modified Eagle’s Medium; FBS, fetal bovine serum; PDI, polydispersity index; Ptx, paclitaxel; RPMI, Roswell Park Memorial Institute; SPION, superparamagnetic iron oxide nanoparticles; SPIONLA-HSA, lauric acid- and human serum albumin-coated SPIONs; SPIONLA-HSA-Ptx, SPIONLA-HSA functionalized with paclitaxel; T, tesla.