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. 2018 Dec 26;13(12):e0209577. doi: 10.1371/journal.pone.0209577

Fig 3. The UV-induced switch of cold-evoked behaviors requires class IV and Ch peripheral sensory neurons.

Fig 3

(A-C) Larvae expressing an active or inactive (control) form of the tetanus toxin transgene (see materials and methods) in class III (CIII), class IV (CIV) or chordotonal neurons (Ch) were tested for cold-evoked behaviors 24 hours after UV exposure. Percent of (A) CT, (B) US, or (C) BR responders were averaged and UV- versus mock-treated responses were compared. n = 90. (D-E) Representative in vivo confocal microscopy images of (D) mock treated larvae and (E) larvae 24 hours post UV treatment. (D-E) Larval chordotonal morphology was visualized via iav-Gal4>UAS-mCD8::GFP. Scale bar: 20 μm. (F) Mean fluorescence intensity normalized to area for cell body and dendrites, where n = 29–30 neurons. Data are presented as the average ± s.e.m (A-C,F). Stats: (A-C) two-tailed Fisher’s Exact test * = p < 0.05. (F) Two-tailed t-test, n.s. = not significant, comparisons were between UV and mock control.