Fig 2. The CTCF sites block crosstalk between the iab-4 and iab-5 domains.
(A) Molecular maps of the Mcp boundary. The coordinates of the McpattP deletion and McpPRE McpCTCF replacement fragments according to complete sequence of BX-C in SEQ89E numbering [4] are shown below. DNAse hypersensitive site is shown as a light gray box above the coordinate bar. Binding sites for GAF, Pita and dCTCF are indicated by blue, red and purple ovals, respectively. PRE element from iab-5 is marked as a blue stripe. Replacement fragments are shown below. wt* indicate wt with respect to Mcp. (B) The cuticle preparations of wt, McpPRE and McpCTCF males. The morphology of the 2nd to 6th abdominal segments is shown. Abnormalities in segment phenotype are shown by the red arrows. The localization of trichomes on the 4th to 6th abdominal tergites are shown in dark field.