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. 2018 Nov 25;38:100–112. doi: 10.1016/j.ebiom.2018.11.004

Fig. 3.

Fig. 3

Cav1 is a direct target for miR-1246. a. The sequences for the 3’UTR region of Cav1 and miR-1246. A firefly luciferase assay was used to show direct targeting of miR-1246 to Cav1. Luciferase activity in HeyA8 and SKOV3-ip1 cells co-transfected with a wild-type (WT) Cav1 transcript and with CTL miRNA mimic or miR-1246 mimic. Data were normalized to Renilla luciferase activity. E.V, Cav1 vector. b. Cav1 protein expression level in normal human ovarian surface epithelial cells (HIO180) and an OC panel (HeyA8, HeyA8-MDR, SKOV3-ip1, SKOV3-TR). c. A negative correlation was seen between miR-1246 level and Cav1 protein level for chemotherapy-sensitive (HeyA8, SKOV3-ip1) and -resistant OC cells (MDR, TR). Resistant and sensitive cancer cells clustered separately. d. Cav1 levels were increased using lentiviral transfection in HeyA8 and SKOV3-ip1 OC cells. e. Treatment with miR-1246 mimic decreased the levels of Cav1 in CTL lentiviral-transfected SKOV3-ip1 OC cells.