Fig. 3.

Overexpression of full length APP and sAPPα enhanced breast cancer proliferation and migration. (A) Expression of full length or sAPPα promoted proliferation in breast cancer cell line (MDA-MB231). MDA-MB231 was transfected with full length (FL), sAPP or control (CTR) construct and were seeded (1 × 10³) in a 96 well plate. Western blotting analysis for APP expression after transfection was shown (insert). Cell proliferation was measured at day 4 by CCK8 assay. (mean ± SD; n = 3). Statistical analysis was performed using student t-test. ***p < 0.001. (B) APP overexpression enhanced migration of breast cancer. Cells transfected with different APP constructs (1 × 10⁵) were seeded with 0.2 ml serum free medium in the upper chamber of Boyen chamber after overnight serum starvation. 0.5 ml medium supplemented with 10% FBS was added to the lower chamber. Migration was evaluated for 22 h. (mean ± SD; n = 3). Statistical analysis was performed using student t-test. ***p < 0.001. All experiments were performed at least three times.