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. 2018 Nov 20;38:89–99. doi: 10.1016/j.ebiom.2018.11.012

Fig. 5.

Fig. 5

Effect of ADAM10 knockdown in breast cancer partly reversed by sAPPα expression. (A) ADAM10 knockdown reduced proliferation in breast cancer cell line (MDA-MB231). MDA-MB231 was transfected with ADAM10 siRNA and were seeded (1 × 103) in a 96 well plate. Cell proliferation was measured at day 4 by CCK8 assay. (mean ± SD; n = 3). Statistical analysis was performed using student t-test. *p < 0.05. (B) ADAM10 knockdown reduced migration of breast cancer. Cells transfected ADAM10 siRNA (1 × 105) were seeded with 0.2 ml serum free medium in the upper chamber of Boyen chamber after overnight serum starvation. 0.5 ml medium supplemented with 10% FBS was added to the lower chamber. Migration was evaluated for 22 h. (C) ADAM10 knockdown effect can be reversed by sAPP overexpression. MDA-MB231 cells co-transfected with ADAM10 siRNA and sAPPα/full length (FL) construct were seeded (1 × 105) with 0.2 ml serum free medium in the upper chamber of Boyen chamber after overnight serum starvation. 0.5 ml medium supplemented with 10% FBS was added to the lower chamber. Migration was evaluated for 22 h. (mean ± SD; n = 3). Statistical analysis was performed using student t-test. ***p < 0.001; **p < 0.01; *p < 0.05; ns-no statistical significance. All experiments were performed at least three times.