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. 2018 Dec 26;6(24):e13962. doi: 10.14814/phy2.13962

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© 2018 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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(A) Expression of chemerin receptors, CMKLR1 and CCRL2, in HUVECs. Chemerin receptors were identified by Western blot analyses using anti‐human CMKLR1 and CCRL2 antibodies. (B) The phosphorylation of Akt and ERK by chemerin. Chemerin stimulation (10 nmol/L) was conducted for the indicated time. The results of one of three experiments with similar results are shown. *P < 0.01 versus control. (C) Confocal immunofluorescence analysis of HUVECs treated with chemerin or VEGF using rabbit mAb against phospho‐Akt (Ser473) and phospho‐ERK (Thr202/Tyr204) (green). Actin filaments have been labeled with Alexa Fluor 568‐conjugated phalloidin (red). Blue pseudo color = DAPI.