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. 2018 Dec 26;6(24):e13962. doi: 10.14814/phy2.13962

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© 2018 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Migration of HUVECs stimulated by chemerin assessed by cell scratch assay. (A) Modified wound healing assay was performed with chemerin (10 nmol/L) or VEGF (5 nmol/L) as chemoattractant. Chemerin stimulation (10 nmol/L) was conducted for the indicated time. After preincubation with LY294002 (1 μmol/L) or PD98059 (5 μmol/L), cells were incubated with chemerin (10 nmol/L). (B) The change rate of width between the leading edge covered by cells before and after 8 h of incubation was quantified. Seven random wounds per each well were quantified. All assays were performed in triplicate. *P < 0.05 versus serum‐free control.