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. 2018 Dec 26;6(24):e13962. doi: 10.14814/phy2.13962

Figure 5.

Figure 5

Chemerin promotes proliferation of HUVECs. Cell growth was arrested using serum‐free EBM‐2 medium for 12 h. After preincubation with LY294002 (1 μmol/L) or PD98059 (5 μmol/L), cells were incubated with chemerin (10 nmol/L) or VEGF (5 nmol/L). (A) Cell proliferation was assessed by counting cells with a hemocytometer. For cell counting, HUVECs were detached from the each seven plates and counted using a hemocytometer. All assays were performed in triplicate. (B) Cell proliferation was assessed by the MTT assay. MTT reduction was read by each 10 wells at 550 nm using a spectrophotometer. All assays were performed in triplicate. The results are shown as the mean ± SE. *P < 0.05 versus control.