Figure 4.

Decidual IGFBP-1 and CK2 colocalization is increased in IUGR, as demonstrated by PLA. (A–E) PLA signals demonstrating the potential interaction of CK2β with total IGFBP-1 in the BPD region of placenta from the AGA group [(A), (C), and (E)] and the IUGR [(B), (D), and (F)] group. A positive PLA signal appears as red punctate spots denoted by white arrows, and DAPI nuclear counterstain (blue) with tissue autofluorescence (pseudo green) is used to show tissue morphology. Pink, dashed, outlined regions in (A) and (B) are shown zoomed in, in (C) and (D). Negative controls are shown in (E) and (F). Original scale bars, 50 µM.