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. 2018 Nov 16;38:69–78. doi: 10.1016/j.ebiom.2018.11.009

Fig. 2.

Fig. 2

CHAF1A promotes cell proliferation of GC. a Correlation of Ki-67 with CHAF1A mRNA expression in 45 pairs of GC and adjacent non-tumor tissues. The data were derived from the GEO database (GSE63089). b CHAF1A mRNA levels in indicated cells transfected with CHAF1A siRNA or CHAF1A over-expression plasmid. c Representative images (left) and quantification (right) of Negative Control (NC) – or CHAF1A siRNA-transfected HGC-27 and MGC-803 cells analyzed in a colony formation assay. d Representative images (left) and quantification (right) of vector- or CHAF1A plasmid-transfected BGC-823 and SGC-7901 cells analyzed in a colony formation assay. e-f The effect of CHAF1A knockdown on cell viability determined by CCK8 assay in HGC-27 and MGC-803 cells. g-h The effect of CHAF1A overexpression on cell viability determined by CCK8 assay in BGC-823 and SGC-7901 cells. Similar results were repeated in three independent experiments. i-k The effect ectopic CHAF1A expression on tumor formation ability in nude mice xenograft model (i), tumor growth curve (j) and tumor weight (k) in each group. *p < 0.0 5, **p < 0.01, ***p < 0.001 by Student's t-test.