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. 2018 Nov 16;38:69–78. doi: 10.1016/j.ebiom.2018.11.009

Fig. 6.

Fig. 6

CHAF1A expression induced by H. pylori is Sp1 dependent. a CHAF1A mRNA expression in six pairs of H. pylori-infected and H. pylori non-infected gastric tissue. The data were derived from the GEO database (GSE27411). b IHC staining for CHAF1A was performed on H. pylori non-infected or H. pylori-infected AG samples. Scale bars: 200 μm (insets 50 μm). c The mRNA levels of CHAF1A in H. pylori- infected AG samples (n = 18) and H. pylori non-infected (n = 73). d-e RT–PCR analysis of CHAF1A mRNA in H. pylori-infected AGS (d) and BGC-823 (e) cells at different time points. f The protein expression of CHAF1A in H. pylori -infected AGS and BGC-823 cells at different time points. g–h CHAF1A and Sp1 changes at RNA level with Sp1 siRNA and H. pylori treatment solely or jointly in AGS (g) and BGC-823 (h) cells cells. i Western blot analysis of CHAF1A and Sp1 protein level with Sp1 siRNA and H. pylori treatment solely or jointly in AGS and BGC-823 cells. j Proposed schematic model of oncogenic function of CHAF1A in gastric cancer.