Figure 1.

Representative dot-plots illustrating how hemorrhagic shock increased the percentage of PD-1⁺ Ly6G⁺ neutrophils in peripheral blood (A,B) and PD-L1⁺ CD31⁺ endothelial cells in lung tissue (C,D). Summary data document that percentage of PD-1⁺ Ly6G⁺ neutrophils increases early and significantly at 6 h and continues to increase over the 24 h following Hem (E). In contrast, the percentage PD-L1⁺ CD31⁺ endothelial cells increase at 6 h post-Hem but decreases by 24 h post-Hem (F). Mean ± SEM, N = 3/group, *p < 0.05 vs. Sham Hem; ^#p < 0.05 vs. 6 h. One-way ANOVA and a Tukey's multiple comparisons test.