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. 2018 Dec 20;5:356. doi: 10.3389/fmed.2018.00356

Figure 3.

Figure 3

Prior exposure to 20% O2 restores the induction of ECFC colony formation in 1% O2. The graph depicts enumeration of ECFC colony outgrowth from umbilical cord blood-derived MNCs. (A) The freshly isolated CB-MNC fractions of three donors were seeded and exposed for varying periods (0–96 h) to ambient oxygen (20% O2) and subsequently transferred to 1% O2 atmosphere for additional culture (B) ECFC colony outgrowth from umbilical cord blood-derived MNCs obtained from three different donors after 24, 48, 72, or 96 h exposure to 20% O2. Cells cultured only in 20% O2 (20%), or 1% O2 (0 h) served as controls. ECFC colonies were quantified when the colonies had become visible in the culture in 20% O2 and expressed as average number ± SEM (n = 3) of counted colonies. Statistical significance was determined by a One-way ANOVA with Bonferroni post-hoc test; *p < 0.05.